一、課程說明(Course Description)
<br>The aim of this course is to provide its participants some basic techniques of molecular biology. 
<br>LS482101 Wed 13:10-17:00
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<br>二、指定用書(Text Books)
<br>Protocols in hard copy will be provided to the participants.
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<br>三、參考書籍(References)
<br>Molecular Biology. Weaver, R.F. 2002, WCB MaGraw-Hill
<br>Molecular Cloning: A Laboratory Manual.3rd Ed. edited by Joseph Sambrook, David W. Russell, 2001. Cold Spring Harbor Laboratory Press.
<br>Current protocols in molecular biology. edited by Frederick M. Ausubel ... [et al.], 2001.John Wiley & Sons, Inc.
<br>Molecular microbiology laboratory: A Writing Intensive Course. Walt Ream ... [et al.], 2003.
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<br>四、教學方式(Teaching Method)
<br>課程由張大慈教授及藍忠昱教授於教室（LSII-330）進行講解; 再由助教帶領於實驗教室(LSII-321)實地操作; 教材：實驗講義
<br>The class will have lecture section given by Professors D.T. Chang and C.Y. Lan before the experimental hours which will be guided by teaching assistants.
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<br>五、教學進度(Syllabus)
<br>01. Check in and equipment introduction 
<br>02. Introduction of bacteria culture and yeast culture
<br>03. Small scale preparation of bacterial plasmid DNA
<br>04. Isolation of yeast genomic DNA
<br>05. 國慶日
<br>06. Isolation of yeast total RNA and reverse transcription 
<br>    (Synthesis of the first strand cDNA)
<br>07. Restriction digestion of DNA
<br>    Agarose gel electrophoresis：Bacterial plasmid DNA, yeast genomic DNA 
<br>    and total RNA
<br>08. Polymerase Chain Reaction
<br>    Isolation and purification of PCR product
<br>09. Preparation of cloning vector and insert
<br>    Purification of cloning vector and insert 
<br>    Spectroscopic analysis and construction of recombinant DNA
<br>10. 全校運動大會(停課)
<br>11. Competent E. coli host cells 
<br>    Transformation - Calcium Chloride method and Electroporation
<br>12. Characterization of recombinant DNA
<br>13. Protein expression
<br>14. SDS-polyacylamide gel electrophoresis
<br>15. Presentation and Proposal report
<br>16. Final exam & check out
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<br>六、成績考核(Evaluation)
<br>1. Laboratory report          30%
<br>2. Final examination          30%
<br>3. Lab performance            20%
<br>4. Presentation and proposal  20% 
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<br>七、可連結之網頁位址
<br>classroom
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