COPIED FROM 09310LS 282100細胞生物學實驗 BY SYSTEM!!! 
<br>
<br>
<br>一、課程說明(Course Description)
<br>學習運用細胞生物學之知識 學習如何用實驗來驗證或發現細胞的生命現象
<br>To learn the basic technique and methods in cell biology and to further apply the knowledge of cell biology to design experiments
<br>
<br> 二、指定用書(Text Books)
<br>
<br>Handouts
<br>
<br> 三、參考書籍(References)
<br>
<br>Morgan, K. S. 2000. Current Potocols in Cell Biology, Wiley-Liss, New York
<br>Freshney, R. I. 2000. Culture of Aminal Cells. A Manual of Basic Techniques, 4th ed. Wiley-Liss, New York
<br>Baker K. 1998. At the Bench. A laboratory navigator, Cold Spring Harbor Laboratory Press.
<br>Research papers (will be delievered)
<br>
<br> 四、教學方式(Teaching Method)
<br>
<br>(1) 練習設計實驗 (如實驗目的 實驗背景 所需儀器設備 材料 預期結果)
<br>(2) 在實驗室實際動手操作 以驗證自己之實驗設計
<br>(3) 討論檢討 修正實驗設計
<br>
<br>
<br> 五、教學進度(Syllabus)
<br>
<br>Part II.  Mammalian Cell Culture --Prof. Linyi Chen 陳令儀
<br>
<br>Lecture 
<br>Mammalian Cell Culture 
<br>- Introduction and basic technique 
<br>Introduction of cell culture room
<br>
<br>Laboratory
<br>Sterile techniques, medium preparation
<br>Subculturing cells, cell counting
<br>Culturing cells, freezing and thawing cells 
<br>Immunostaining actin cytoskeleton structure of fibroblast cells and visualized by fluorescence microscopy
<br>Immunostaining actin cytoskeleton structure of neuron cells and visualized by fluorescence microscopy
<br>
<br>
<br> 六、成績考核(Evaluation)
<br>
<br>For PART II (Chen's part), we will have lab reports and in-class quiz. 
<br>Final exam will be presentation of experimental results.
<br>Your grade will depend on the lab report, lab performance, quiz and presentation.
<br>
<br>葉世榮
<br>週數	週二	週三	主題	備註	
<br>1	2/19	2/20	神經科學基本概念		
<br>2	2/26	2/27	細胞的電路概念與model cell 電阻測量		
<br>3	3/4	3/5	螯蝦神經構造與解剖		
<br>4	3/11	3/12	電生理儀器設備概念與使用(一)		
<br>5	3/18	3/19	電生理儀器設備概念與使用(二)		
<br>6	3/25	3/26	膜電位測量 resting membrane potential		
<br>7	4/1	4/2	停課，校際活動週（春假）		
<br>8	4/8	4/9	興奮性神經電位與動作電位		
<br>9	4/15	4/16	實驗成果驗收		
<br>
<br>陳令儀
<br>10	4/22	4/23	Lecture: 介紹助教, 生圖看錄影帶, 儀器, 器材, 實驗室 
<br>                           介紹, 學期評分說明		
<br>			Lab: Clean culture room, 練習使用pipetaid, 
<br>                            pipetman, 練習sterile techniques		
<br>11	4/29	4/30	Lecture: 介紹光學顯微鏡(助教), 介紹動物細胞培養 (國
<br>                           衛院研究員 林嬪嬪博士)		
<br>			Lab: 配製培養基, 觀察細胞, feed cells	
<br>12	5/6	5/7	Lecture: Methods for cell counting and freezing 
<br>                           cells		
<br>			Lab: 觀察細胞, 分細胞, 細胞計數, 凍細胞	
<br>13	5/13	5/14	Lecture: Introducing actin cytoskeleton structure 
<br>                           of cells and actin staining technique, introducing 
<br>                           immunofluorescence microscopy and the usage 
<br>                           of fluorescence microscope (助教)		
<br>			Lab: 解凍細胞, 觀察細胞		
<br>14     5/20-5/24 5/21-5/24	Lecture:小考(90 min)		
<br>			Lab: Staining actin cytoskeleton structure of COS-7 
<br>                            cells with rhodamine phalloidin, taking images 
<br>                            (please make reservation ahead of time and show up 
<br>                            to use the microscope on time)		
<br>15     5/27-5/31 5/28-5/31	Lab: Staining actin cytoskeleton structure of 
<br>                            neuronal cells (differentiated PC12 cells) with 
<br>                            rhodamine phalloidin, taking images		
<br>16	6/3	6/4	Final exam (1h)		
<br>			Presentation of actin staining results	
<br>17	6/10	6/11	課程移至5/22-5/24		
<br>18	6/17	6/18	課程移至5/29-5/31	
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>