一、課程說明 (Course Description)
教授共軛焦顯微鏡之原理與技術，包括生物標本製備，共軛焦顯微鏡操作與3D生物影像處理等。將安排專事顯微鏡操作及影像處理之軟、硬體工程師與老師共同指導。
二、指定用書 (Text Books)
Alan R. Hibbs (2004) Confocal Microscopy for Biologists. Amazon
三、參考書籍 (References)
1.Alberto Diaspro (2002) Confocal and Two-Photon Microscopy : Foundations, Applications and Advances
2.Brian Matsumoto (2002) Cell Biological Applications of Confocal Microscopy, Second Edition (Methods in Cell Biology)
3.Beesley, J. E. (1993) Immunocytochemistry, A Practical Approach. IRL press.
4.Lacey, A. J. (1989) Light Microscopy in Biology, A Practical Approach. IRL press, New York.
5.Slayter, E. M. and Slayter H. S. (1992) Light and Electron Microscopy. Cambridge University Press, New York.
6.Stevens, J.K., Milles, L.R. and Trogadis, J. E. (1994) Three-dimensional confocal microscopy of biological specimens. London, Academic Press.
7.Taylor, D. L. and Wang, Y.-L. and (1989) Fluorescence Microscopy of Living Cells in Culture, Part B. Quantitative Fluorescence Microscopy Imaging and Spectroscopy. Academic Press. New York.
8.Wang, Y.-L. and Taylor, D. L. (1989) Fluorescence Microscopy of Living Cells in Culture, Part A. Fluorescent Analogs, Labeling Cells, and Basic Microscopy. Academic Press. New York.
四、教學方式 (Teaching Method)
Interactive lecture, discussion, practical training
五、教學進度 (Syllabus)
9/15 Overview of confocal microscopy (11:10-12:00，江安世教授)
9/22 Light and fluorescence microscopy: principle & operation (台灣儀器工程師)
9/29 Confocal microscopy: principle (台灣儀器工程師)
Submit the name list of your group (4 persons/group)
10/6 Confocal microscopy: operation (台灣儀器工程師)
10/13 Guideline for using light and confocal microscopes
*FocusClear technology and multicolor imaging (林奇文)
10/13-17 2-hour practice for each group (林奇文)
10/20 *Imaging gene expression patterns reported by GFP (林暉皓)
10/20-24 2-hour practice for each group (林暉皓)
10/27 3D image visualization by Amira software (擎曄科技工程師)
11/3 Term paper presentation (江安世教授)
六、成績考核 (Evaluation)
Discussion 20%
Practice 40%
Term paper 40%
七、可連結之網頁位址
http://brc.life.nthu.edu.tw/Courses-Meetings.htm