COPIED FROM 09310LS 282100細胞生物學實驗 BY SYSTEM!!! 
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<br>一、課程說明(Course Description)
<br>學習運用細胞生物學之知識 學習如何用實驗來驗證或發現細胞的生命現象
<br>To learn the basic technique and methods in cell biology and to further apply the knowledge of cell biology to design experiments
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<br> 二、指定用書(Text Books)
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<br>Handouts
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<br> 三、參考書籍(References)
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<br>Morgan, K. S. 2000. Current Potocols in Cell Biology, Wiley-Liss, New York
<br>Freshney, R. I. 2000. Culture of Aminal Cells. A Manual of Basic Techniques, 4th ed. Wiley-Liss, New York
<br>Baker K. 1998. At the Bench. A laboratory navigator, Cold Spring Harbor Laboratory Press.
<br>Research papers (will be delievered)
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<br> 四、教學方式(Teaching Method)
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<br>(1) 練習設計實驗 (如實驗目的 實驗背景 所需儀器設備 材料 預期結果)
<br>(2) 在實驗室實際動手操作 以驗證自己之實驗設計
<br>(3) 討論檢討 修正實驗設計
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<br> 五、教學進度(Syllabus)
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<br>Part II.  Mammalian Cell Culture --Prof. Linyi Chen 陳令儀
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<br>Lecture 
<br>Mammalian Cell Culture 
<br>- Introduction and basic technique 
<br>Introduction of cell culture room
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<br>Laboratory
<br>Sterile techniques, medium preparation
<br>Subculturing cells, cell counting
<br>Culturing cells, freezing and thawing cells 
<br>Immunostaining actin cytoskeleton structure of fibroblast cells and visualized by fluorescence microscopy
<br>Immunostaining actin cytoskeleton structure of neuron cells and visualized by fluorescence microscopy
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<br> 六、成績考核(Evaluation)
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<br>For PART II (Chen's part), we will have lab reports and in-class quiz. 
<br>Final exam will be presentation of experimental results.
<br>Your grade will depend on the lab report, lab performance, quiz and presentation.
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<br>97下 細生實驗 課程進度表 
<br>週數 週二 週三 主題 備註 
<br>1 2/24 2/25 神經科學基本概念 葉世榮 
<br>2 3/3 3/4 細胞的電路概念與model cell 電阻測量 
<br>3 3/10 3/11 傾聽神經衝動 即席報告 
<br>4 3/17 3/18 螯蝦神經構造與解剖 
<br>5 3/24 3/25 電生理儀器設備概念與使用 
<br>6 3/31 4/1 停課，校際活動週（春假） 
<br>7 4/7 4/8 膜電位測量 resting membrane potential 
<br>8 4/14 4/15 興奮性神經電位與動作電位 
<br>9 4/21 4/22 實驗成果驗收 
<br>10 4/28 4/29 Lecture: 介紹助教, 生圖看錄影帶, 儀器, 器材, 實驗室介紹, 學期評分說明 陳令儀 
<br>Lab: Clean culture room, 練習使用pipetaid, pipetman, 練習sterile techniques 
<br>11 5/5 5/6 Lecture: 介紹光學顯微鏡(助教), 介紹動物細胞培養 (陳令儀) 
<br>Lab: 配製培養基, 觀察細胞, feed cells 
<br>12 5/12 5/13 Lecture: Methods for cell counting and freezing cells 
<br>Lab: 觀察細胞, 分細胞, 細胞計數, 凍細胞 
<br>13 5/19 5/20 Lecture: Introducing actin cytoskeleton structure of cells and actin staining technique (陳令儀), introducing immunofluorescence microscopy and the usage offluorescence microscope (助教) 
<br>Lab: 解凍細胞, 觀察細胞 
<br>14 5/26-5/30 5/27-5/30 Lecture:小考(90 min) 5/28端午節 
<br>Lab: Staining actin cytoskeleton structure of COS-7 cells with rhodamine phalloidin, taking images (please make reservation ahead of time and show up to use the microscope on time) 
<br>15 6/2-6/6 6/3-6/6 "Lab: Staining actin cytoskeleton structure of neuronal cells (differentiated 
<br>PC12 cells) with rhodamine phalloidin, taking images" 
<br>16 6/9 6/10 Final exam (1h) 
<br>Presentation of actin staining results 
<br>17 6/16 6/17 課程移至5/28-5/30 
<br>18 6/23 6/24 課程移至6/4-6/6 期末考週 
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