果蠅神經解剖學
(Drosophila
Neuroanatomy)
課程大綱
課程代號：09810LSSN510200
任課老師：江安世教授
上課時間：週一
AM
10:10-12:00
上課地點：生科二館113教室
一、課程說明
(Course
Description)
教授果蠅神經解剖學之相關研究技術，包括各種果蠅神經標幟技術、神經組織製備，共軛焦
顯微鏡技術與3D神經影像處理等，各項技術並有實作練習。希望藉此課程能使修課同學對果蠅神
經影像技術有一整體了解與實作經驗。
二、指定用書
(Text
Books)
1. Chiang
AS,
Lin
CY,
Chuang
CC,
Chang
HM,
Hsieh
CH
et
al.
(2009)
Interactive
three-dimensional
wiring
“connectome”
diagram
of
the
Drosophila
brain.
(submitted)
2. FlyCircuit
(http://140.110.17.72/flycircuit_work/)
三、參考書籍
(References)
1. Nicholas
J.
Strausfeld
(1976)
Atlas
Of
An
Insect
Brain.
Springer-Verlag,
New
York.
2. Alan
R.
Hibbs
(2004)
Confocal
Microscopy
for
Biologists.
Amazon.
3. Zeiss
LSM710
operation
manu.
4. Flybrain
(http://flybrain.neurobio.arizona.edu/)
四、教學方式
(Teaching
Method)
Interactive
lecture,
discussion,
practical
training
五、課程大綱
(Outline)
9/20
Introduction
(江安世教授)
Section
I:
Gross
anatomy
of
Drosophila
central
nervous
system
9/27
Fundamental
neuroanatomy
of
Drosophila
(江安世教授)
Light
and
fluorescence
microscopy:
principle
&
operation
(林奇文)
10/4
Confocal
microscopy:
principle
&
operation
(practice
with
IHC
samples,
林奇文)
Immunohistochemistry
(IHC)
and
FocusClear
technology
(with
practice,
林宣文)
10/11
Brainbow
imaging
(with
practice,
林暉皓)
10/18
First
oral
report
and
discussion
Section
II:
Anatomy
of
neural
circuits
10/25
Single
neuron
labeling:
mosaic
analysis
with
a
repressible
cell
marker
(MARCM)
and
Immunohistochemistry
(IHC)
(with
practice,
施孟甫)
11/1
Visualization
of
neurons
and
their
polarity
with
syt::HA/Dscam::GFP
(with
practice,
石翔文)
11/8
Visualization
of
neuronal
connectivity
with
GRASP
(with
practice,
林志勇)
11/15
Tracing
neural
tracts
with
photoactivatable
GFP
(PaGFP)
(with
practice,
鄭富元)
Section
III:
Function
of
neural
circuits
11/22
Monitoring
protein
synthesis
with
photoconvertible
fluorescent
protein
Kaede
(with
practice,
陳俊朝)
11/29
Functional
imaging
of
neural
activity
with
GCaMP
(黎思宇)
12/6
Practice
with
GCaMP-labeled
samples
(黎思宇)
12/13
Second
oral
report
and
discussion
Section
IV:
Analysis
of
neural
network
12/20
3D
image
processing:
segmentation,
warping
and
visualization
(擎曄有限公司)
12/27
Practice:
3D
image
processing
(王定遠)
01/03
FlyCircuit
database:
image
browsing
and
combinatorial
visualization
(張修明博士)
01/10
Third
oral
report
and
discussion
六、成績考核
(Evaluation)
Discussion
20%
Practice
40%
Term
paper
40%
七、可連結之網頁位址
http://brc.life.nthu.edu.tw/html/course_meeting.html
Drosophila
neuroanatomy
Virtual
Insect
Brain
is
a
brief
course
of
neuroanatomy
of
Drosophila
melanogaste.
It
also
provides
nomenclature
for
brain
anatomy.
Flybase
is
a
database
of
genetic
and
molecular
data
for
Drosophila.
FlyBase
includes
data
on
all
species
from
the
family
Drosophilidae;
the
primary
species
represented
is
Drosophila
melanogaster.
Berkeley
Drosophila
Genome
Project
(BDGP).
The
goals
of
the
Drosophila
Genome
Center
are
to
finish
the
sequence
of
the
euchromatic
genome
of
Drosophila
melanogaster
to
high
quality,
and
to
generate
and
maintain
biological
annotations
of
this
sequence.
The
Bloomington
Fly
Stock
Center
collects,
maintains
and
distributes
Drosophila
melanogaster
strains
for
research.
The
Basic
Atlas
of
the
Drosophila
Brain
provides
the
user
with
a
hypertext
tour
guide
to
the
basic
structural
elements
of
the
Drosophila
nervous
system.
BLASTR
(Basic
Local
Alignment
Search
Tool)
is
a
set
of
similarity
search
programs
designed
to
explore
all
of
the
available
sequence
databases
regardless
of
whether
the
query
is
protein
or
DNA.
The
Baylor
College
of
Medicine
(BCM)
Search
Launcher
is
an
ongoing
project
to
organize
molecular
biology-related
search
and
analysis
services
available
on
the
web
by
function,
by
providing
a
single
point-of-entry
for
related
searches.
Pfam
is
a
large
collection
of
multiple
sequence
alignments
and
hidden
Markov
models
covering
many
common
protein
families.
Prodom
is
a
comprehensive
set
of
protein
domain
families
automatically
generated
from
the
SWISS-PROT
and
TrEMBL
sequence
databases.
The
ExPASy
(Expert
Protein
Analysis
System)
proteomics
server
of
the
Swiss
Institute
of
Bioinformatics
is
dedicated
to
the
analysis
of
protein
sequences
and
structures.