一、課程說明(Course Description): 
<br>The Yeast Genetics and Genomics course is designed to teach students various approaches needed to dissect complex problems in budding yeast Saccharomyces cerevisiae. Classical genetic approaches, such as the isolation and characterization of mutants, complementation, tetrad analysis, and mitotic recombination will be emphasized. Molecular genetic techniques, including yeast transformation, gene replacement with plasmids and PCR, construction and analysis of gene fusions, and generation of mutations in cloned genes, will also be addressed. Furthermore, modern cytological approaches such as epitope tagging and imaging yeast cells using indirect immunofluorescence, GFP-protein fusions and a variety of fluorescent indicators for various subcellular organelles will be covered in this course. These classical, molecular and cytological yeast approaches will benefit students to gain insights in genetic suppression and synthetic lethality in order to identify and interpret various kinds of genetic interactions. Latest papers on fundamental aspects of yeast genetics will also be discussed along with the corresponding lectures.
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<br>二、指定用書(Text Books): N/A
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<br>三、參考書籍(References): 
<br>Methods in Yeast Genetics. 
<br>David C. Amberg, Daniel J. Burke, Jeffery N. Strathern.
<br>CSHL Press, 2005
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<br>四、教學方式(Teaching Method): 
<br>English lecture combined with latest journal paper discussion.
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<br>五、教學進度(Syllabus):
<br>02/25 Week 1: Introduction of the course and yeast
<br>03/04 Week 2: N/A
<br>03/11 Week 3: S.cerevisiae as an experimental model
<br>03/18 Week 4: Isolation of yeast mutants
<br>03/25 Week 5: Mitotic recombination and random spore analysis 
<br>04/01 Week 6: Transformation of yeast
<br>04/08 Week 7: N/A 
<br>04/15 Week 8: Mid-term 
<br>04/22 Week 9: Epigenetics in yeast 
<br>04/29 Week 10: Manipulation of yeast gene and organelle trafficking
<br>05/06 Week 11: Synthetic lethal mutants
<br>05/13 Week 12: Isolation mutants by insertional mutagenesis
<br>05/20 Week 13: Yeast protein expression 
<br>05/27 Week 14: Yeast two-hybrid system 
<br>06/03 Week 15: Applications of imaging tools in yeast 
<br>06/10 Week 16: From genes to genomics
<br>06/17 Week 17: Useful techniques in yeast system
<br>06/24 Week 18: Final Exam 
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<br>六、成績考核(Evaluation):
<br>35% - Mid-term Report
<br>35% - Final Exam
<br>20% - discussion and written reports
<br>10% - attendance.
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<br>七、可連結之網頁位址: N/A
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