一、課程說明(Course Description)
In this course we will learn: 1) recombinant DNA technology; 2) procedure of
generation of transgenic and gene knock mice; 3) the molecular mechanism of gene
gain/loss of function in mouse transgenic model; 4) conventional and conditional
transgenic gene expression models; 5) conventional and conditional gene KO models
and Cre-Lox technology; 6) applications of mouse models in developmental biology
research; 7) established mouse models in human disease research. We will review
about how transgenic animals are generated and what recombinant DNA technologies
are involved. The well-established disease models will be introduced in class and
discussed, including the rationale and application of transgenic models in
developmental biology, physiology and disease related research.
二、指定用書(Text Books)
Selected papers
三、參考書籍(References)
(1)Methods in Enzymology Vol. 476. Guide to Techniques in Mouse Development, Part
A: Mice, Embryos, and Cells, 2nd Edition. Edited by Paul M. Wassarman and
Philippe M. Soriano.
(2)Regulation of Gene Expression: Molecular Mechanisms. Gary H. Perdew, John P.
Vanden Heuvel, and Jeffrey M. Peters. 2006.
四、教學方式(Teaching Method)
Lecture/ Seminar /Discussion
五、教學進度(Syllabus)
9/19 Introduction to transgenic models
9/26 Promoters and tissue specific expression
10/3 TetOn/Off and Tamoxifen inducible system
10/10 (Holiday)
10/17 Technical knockout: Antisense RNAi
10/24 Physical Knockout: Homologous recombination
10/31 Gene targeting in ES cells: CRE-LoxP
11/7 Gene targeting Ink4a locus
11/14 Gene trapping strategy
11/21 (University Game Day)
11/28 Mid-term
12/5 Lung cancer genetics and chemical models
12/12 DEN/Pb model induce liver cancer (HCC)
12/19 Prostate cancer models: Tramp and Lady
12/26 Colorectal cancer model
1/2 Injury and tissue regeneration
1/9 Lineage tracing in Developmental Biology Research
1/16 Final
六、成績考核(Evaluation)
Attendance (10%),
Discussion/Seminar (30%),
Mid-term (30%) and
Final report (30%)