Syllabus for Special Topics on Live Cell Imaging

Lecture-1: Introduction to live cell imaging
-- General introduction
Lecture-2: The choice of microscope setting
 Introduce current modules of conventional microscopes
 Epifluorescence microscope
 Deconvolution microscope
 Confocal-based microscope
Lecture-3: The choice of protein labeling
 Introduce fluorescent proteins
 Novel developed commercial tags for multiple imaging
Lecture-4: Visualize the organelles in live cells
 Organelle markers
 Fluorescence dyes used for living cells
Lecture-5: Cell cycle application on live cell imaging
 Interphase versus mitosis
 Mitotic progression
 Chromosome dynamics
 Cytokinesis
Lecture-6: Monitor intracellular trafficking
 Dynamic analysis of macromolecules
Lecture-7: Monitor protein-protein interaction
 Colocalization
 FRET
 FLIM
Lecture-8: Mid-term exam
Lecture-9: Practical training - set up the experiment and image analysis
Lecture-10: Practical training - cell cycle progression
Lecture-11: Practical training - mitotic progression
Lecture-12: Practical training – endocytosis/phagocytosis
Lecture-13: Practical training – membrane fusion
Lecture-14: Practical training – protein trafficking
Lecture-15: Final Exam