Molecular Biology of the Yeast  酵母菌分子生物學
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<br>一、課程大綱 (Course Description): 
<br>The Molecular Biology of the Yeast is designed to teach students basic molecular biology concepts of yeast, both Saccharomyces cerevisiae and Candida albicans. Except basic biological features of yeast, classical genetic approaches, such as the isolation and characterization of mutants, complementation, tetrad analysis, and mitotic recombination will be emphasized. Molecular genetic techniques, including yeast transformation, gene replacement with plasmids and PCR, construction and analysis of gene fusions, and generation of mutations in cloned genes, will also be addressed. Furthermore, modern cytological and systematical approaches will be covered in this course. These classical, molecular cytological and systematical yeast approaches will benefit students to gain insight information while applying yeast as research model. Latest papers on fundamental aspects of yeast genetics will also be discussed along with the corresponding lectures.
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<br>二、指定用書(Text Books): N/A
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<br>三、參考書籍(References): 
<br>	Genetic Techniques for Biological Research – A case study approach
<br>	Corinne A. Michaels
<br>	John Wiley & Sons, Ltd., 2002
<br>	Methods in Yeast Genetics. 
<br>	David C. Amberg, Daniel J. Burke, Jeffery N. Strathern.
<br>	CSHL Press, 2005
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<br>四、教學方式(Teaching Method): 
<br>Lecture combined with latest journal paper discussion.
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<br>五、教學進度(Syllabus):
<br>Week 1: Introduction of S.cerevisiae
<br>Week 2: Transformation of yeast
<br>Week 3: Mitotic recombination and random spore analysis
<br>Week 4: Isolation of yeast mutants
<br>Week 5: Manipulation of yeast genes
<br>Week 6: Synthetic lethal mutants
<br>Week 7: Genetic screen with insertional mutagenesis
<br>Week 8: Yeast protein expression and two-hybrid system
<br>Week 9: Midterm
<br>Week 10: Genomic perspectives of S. cerevisiae
<br>Week 11: Yeast systems biology
<br>Week 12: Introduction of pathogenic yeast: Candida & Cryptococcus spp.
<br>Week 13: Candida albicans (1)
<br>Week 14: Candida albicans (2) 
<br>Week 15: Non-albicans Candida species
<br>Week 16: Cryptococcus species
<br>Week 17: Final exam
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<br>六、成績考核(Evaluation):
<br>40% - Mid-term Exam
<br>40% - Final Exam
<br>10% - discussion in the classes
<br>10% - attendance.
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<br>七、可連結之網頁位址 (Hyperlink):
<br>All the materials and reports will be upload to NTHU iLMS e-learning system
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