一、課程說明(Course Description)
<br>In this course we will learn: 1) recombinant DNA technology; 2) procedure of 
<br>generation of transgenic and gene knock mice; 3) the molecular mechanism of gene 
<br>gain/loss of function in mouse transgenic model; 4) conventional and conditional 
<br>transgenic gene expression models; 5) conventional and conditional gene KO models 
<br>and Cre-Lox technology; 6) applications of mouse models in developmental biology 
<br>research; 7) established mouse models in human disease research. We will review 
<br>about how transgenic animals are generated and what recombinant DNA technologies 
<br>are involved. The well-established disease models will be introduced in class and 
<br>discussed, including the rationale and application of transgenic models in 
<br>developmental biology, physiology and disease related research.
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<br>二、指定用書(Text Books)
<br>Selected papers
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<br>三、參考書籍(References)
<br>(1)Methods in Enzymology Vol. 476. Guide to Techniques in Mouse Development, Part 
<br>A: Mice, Embryos, and Cells, 2nd Edition. Edited by Paul M. Wassarman and 
<br>Philippe M. Soriano.
<br>(2)Regulation of Gene Expression: Molecular Mechanisms. Gary H. Perdew, John P. 
<br>Vanden Heuvel, and Jeffrey M. Peters. 2006. 
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<br>四、教學方式(Teaching Method)
<br>Lecture/ Seminar /Discussion
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<br>五、教學進度(Syllabus)
<br>2/19	Introduction and mouse genetics 
<br>2/26	Principle of mouse transgenic models
<br>3/5     Promoters and tissue specific expression
<br>3/12    TetOn/Off and Tamoxifen inducible system
<br>3/19	Technical knockout: Antisense RNAi
<br>3/26	Physical Knockout: Homologous recombination
<br>4/2   	Gene targeting in ES cells: CRE-LoxP system
<br>4/9 	Gene targeting strategy: Ink4a locus
<br>4/16	Gene trapping strategy
<br>4/23	Lineage tracing in Developmental Biology
<br>4/30    Mid-term exam
<br>5/7 	Genetic and chemical induced lung cancer models
<br>5/14	DEN/Pb model induce liver cancer (HCC)
<br>5/21	Prostate cancer models: Tramp and Lady
<br>5/28	Colorectal cancer model
<br>6/4 	Injury and tissue regeneration
<br>6/11    Mouse models in Neuroscience
<br>6/18    Final report
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<br>六、成績考核(Evaluation)
<br>Attendance (10%), Discussion/Seminar (30%),Mid-term (30%) and Final report (30%).
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