一、課程說明	
<br>This course will cover several modern biophysical tools for protein-ligand interactions. These tools include isothermal titration calorimetry (ITC), scanning differential calorimetry (SDC), surface plasmon resonance (SPR), fluorescence spectroscopy, and fluorescence single-molecule measurements. The scope of this class is not to focus on the details on the theory of each method, but to emphasize the advantages and limitations of each technique. After taking this course, students will be capable to use the appropriate methods for practical research design.
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<br>二、指定用書	
<br>Biophysical Tools for Biologists, Volume One: In Vitro Techniques. Edited by Dr. John J. Correia and Dr. H. William Detrich, III (2008) ISBN: 978-0-12-372520-2 
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<br>三、參考書籍	
<br>1. Principles of Fluorescence Spectroscopy. 3rd edition, Joseph R. Lakowicz (2011) ISBN: 0387312781
<br>2. Handbook of Single Molecule Fluorescence Spectroscopy. Christopher Gell, David Brockwell, Alastair Smith (2006) ISBN: 978-0198529422
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<br>四、教學方式	
<br>互動式教學，鼓勵學生提問和發表意見。
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<br>五、教學進度	
<br>March: basic principles of protein-ligand interactions
<br>April: ITC and SDC
<br>May: SPR; basic principles and applications of fluorescence spectroscopy
<br>June: fluorescence single-molecule measurements
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<br>六、成績考核	
<br>課堂參與(20%)，每週期刊口頭報告(40%)，每週作業(40%)
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<br>七、講義位址
<br>    http://	
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