一、課程說明(Course Description)
<br>In this course we will learn: 1) recombinant DNA technology; 2) procedure of 
<br>generation of transgenic and gene knock mice; 3) the molecular mechanism of gene 
<br>gain/loss of function in mouse transgenic model; 4) conventional and conditional 
<br>transgenic gene expression models; 5) conventional and conditional gene KO models 
<br>and Cre-Lox technology; 6) applications of mouse models in developmental biology 
<br>research; 7) established mouse models in human disease research. We will review 
<br>about how transgenic animals are generated and what recombinant DNA technologies 
<br>are involved. The well-established disease models will be introduced in class and 
<br>discussed, including the rationale and application of transgenic models in 
<br>developmental biology, physiology and disease related research.
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<br>二、指定用書(Text Books)
<br>Selected papers
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<br>三、參考書籍(References)
<br>(1)Methods in Enzymology Vol. 476. Guide to Techniques in Mouse Development, Part 
<br>A: Mice, Embryos, and Cells, 2nd Edition. Edited by Paul M. Wassarman and 
<br>Philippe M. Soriano.
<br>(2)Regulation of Gene Expression: Molecular Mechanisms. Gary H. Perdew, John P. 
<br>Vanden Heuvel, and Jeffrey M. Peters. 2006. 
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<br>四、教學方式(Teaching Method)
<br>Lecture/ Seminar /Discussion
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<br>五、教學進度(Syllabus)
<br>9/16	Introduction and principle of transgenic mouse models 
<br>9/23	Promoters and tissue specific expression
<br>9/30    TetOn/Off and Tamoxifen inducible system 
<br>10/7    Technical knockout: Antisense RNAi
<br>10/14	Physical gene targeting (KI and KO): Homologous recombination in ES cells 
<br>10/21	Conditional gene knockout: CRE-LoxP system
<br>10/28   Gene targeting Ink4a locus 
<br>11/04 	Lineage tracing in Developmental Biology Research 
<br>11/11	Gene trapping strategy and Mutagenesis 
<br>11/18	(University Game Day)
<br>11/25   mid-term
<br>12/02 	Genetic and chemical induced lung cancer models
<br>12/09	Prostate cancer models: Tramp and Lady
<br>12/16	Mouse models for pancreatic cancer and diabetes
<br>12/23	Liver cancer (HCC) and Colorectal cancer model
<br>12/30 	Injury and tissue regeneration
<br>01/06   Mouse models in Neuroscience and brain mapping
<br>01/13   Final report
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<br>六、成績考核(Evaluation)
<br>Attendance (10%), Discussion/Seminar (30%),Mid-term (30%) and Final report (30%).
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