Syllabus for Special Topics on Live Cell Imaging

Lecture-1: Introduction to live cell imaging
-- General introduction
Lecture-2: The choice of microscope setting
轩 Introduce current modules of conventional microscopes
轩 Epifluorescence microscope
轩 Deconvolution microscope
轩 Confocal-based microscope
Lecture-3: The choice of protein labeling
轩 Introduce fluorescent proteins
轩 Novel developed commercial tags for multiple imaging
Lecture-4: Visualize the organelles in live cells
轩 Organelle markers
轩 Fluorescence dyes used for living cells
Lecture-5: Cell cycle application on live cell imaging
轩 Interphase versus mitosis
轩 Mitotic progression
轩 Chromosome dynamics
轩 Cytokinesis
Lecture-6: Monitor intracellular trafficking
轩 Dynamic analysis of macromolecules
Lecture-7: Monitor protein-protein interaction
轩 Colocalization
轩 FRET
轩 FLIM
Lecture-8: Mid-term exam
Lecture-9: Practical training - set up the experiment and image analysis
Lecture-10: Practical training - cell cycle progression
Lecture-11: Practical training - mitotic progression
Lecture-12: Practical training – endocytosis/phagocytosis
Lecture-13: Practical training – membrane fusion
Lecture-14: Practical training – protein trafficking
Lecture-15: Final Exam