一、課程說明(Course Description)
透過實驗操作,讓學生熟悉生物化學中各主要分子(如醣類、脂肪、胺基酸和蛋白質等)的特性,以及生化研
究中經常使用到的實驗方法與技術(如透析、電泳、色層分析、酵素的分離與檢測等)。

二、指定用書(Text Books)
生物化學實驗講義(在第一堂課時發給)

三、教學方式(Teaching Method)
學生在老師及助教的指導下﹐依照實驗講義操作實驗 ﹐並繳交報告。

四、教學進度(Syllabus)

Week Experiments
1 Introduction and grouping
2 pH indicators and spectrophotometry
Part I Studies of biomolecules
3 Determination of carbohydrate and vitamin
4 Lipid Aggregation in Water and permeability of membrane
6 Assay of amino acids
7 Isoelectric points and absorption spectra of amino acids
8 Quantification and precipitation of proteins
Part II Basic experimental techniques
9 Dialysis
10 Column chromatography
11 Thin layer chromatography
12 Electrophoresis
Part III Enzyme kinetics
13 Photosynthesis, chloroplast isolation and activity test
14 Characterization of an enzyme, acid phosphatase
15 Kinetic properties of acid phosphatase
16 Final examination; Cleaning and check-out

五、成績考核(Evaluation)

1.Laboratory note/report (40%)
2.Final examination (30%)
3.Lab performance evaluated by teaching assistants (30%)