Syllabus for Special Topics on Live Cell Imaging
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<br>Lecture-1: Introduction to live cell imaging
<br>	      -- General introduction
<br>Lecture-2: The choice of microscope setting 
<br>A533;	Introduce current modules of conventional microscopes
<br>A533;	Epifluorescence microscope
<br>A533;	Deconvolution microscope
<br>A533;	Confocal-ba<x>sed microscope
<br>Lecture-3: The choice of protein labeling
<br>A533;	Introduce fluorescent proteins
<br>A533;	Novel developed commercial tags for multiple imaging
<br>Lecture-4: Visualize the organelles in live cells
<br>A533;	Organelle markers
<br>A533;	Fluorescence dyes used for living cells
<br>Lecture-5: Cell cycle application on live cell imaging
<br>A533;	Interphase versus mitosis
<br>A533;	Mitotic progression
<br>A533;	Chromosome dynamics
<br>A533;	Cytokinesis
<br>Lecture-6: Monitor intracellular trafficking
<br>A533;	Dynamic analysis of macromolecules
<br>Lecture-7: Monitor protein-protein interaction
<br>A533;	Colocalization
<br>A533;	FRET
<br>A533;	FLIM
<br>Lecture-8: Mid-term exam
<br>Lecture-9: Practical training - set up the experiment and image analysis
<br>Lecture-10: Practical training - cell cycle progression
<br>Lecture-11: Practical training - mitotic progression
<br>Lecture-12: Practical training – endocytosis/phagocytosis
<br>Lecture-13: Practical training – membrane fusion
<br>Lecture-14: Practical training – protein trafficking
<br>Lecture-15: Final Exam
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