一、課程說明(Course Desc<x>ription)
<br>In this course we will learn: 1) recombinant DNA technology; 2) procedure of 
<br>generation of transgenic and gene knock mice; 3) the molecular mechanism of gene 
<br>gain/loss of function in mouse transgenic model; 4) conventional and conditional 
<br>transgenic gene ex<x>pression models; 5) conventional and conditional gene KO 
<br>models 
<br>and Cre-Lox technology; 6) applications of mouse models in developmental biology 
<br>research; 7) established mouse models in human disease research. We will review 
<br>about how transgenic animals are generated and what recombinant DNA technologies 
<br>are involved. The well-established disease models will be introduced in class 
<br>and 
<br>discussed, including the rationale and application of transgenic models in 
<br>developmental biology, physiology and disease related research.
<br>
<br>二、指定用書(Text Books)
<br>Selected papers
<br>
<br>三、參考書籍(References)
<br>(1)Methods in Enzymology Vol. 476. Guide to Techniques in Mouse Development, 
<br>Part 
<br>A: Mice, Embryos, and Cells, 2nd Edition. Edited by Paul M. Wassarman and 
<br>Philippe M. Soriano.
<br>(2)Regulation of Gene ex<x>pression: Molecular Mechanisms. Gary H. Perdew, John 
<br>P. 
<br>Vanden Heuvel, and Jeffrey M. Peters. 2006. 
<br>
<br>四、教學方式(Teaching Method)
<br>Lecture/ Seminar /Discussion
<br>
<br>
<br>五、教學進度(Syllabus)
<br>Week 1	Introduction and principle of transgenic mouse models 
<br>Week 2	Promoters and tissue specific ex<x>pression
<br>Week 3   TetOn/Off and Tamoxifen inducible system 
<br>Week 4  Technical knockout: Antisense RNAi
<br>Week 5	Physical gene targeting (KI and KO): Homologous recombination in ES 
<br>cells 
<br>Week 6	Conditional gene knockout: CRE-LoxP system
<br>Week 7   Gene targeting Ink4a locus 
<br>Week 8	Lineage tracing in Developmental Biology Research 
<br>Week 9	(University Game/Sport Day) 
<br>Week 10	Gene trapping strategy and Mutagenesis
<br>Week 11 mid-term
<br>Week 12	Genetic and chemical induced lung cancer models
<br>Week 13 Prostate cancer models: Tramp and Lady
<br>Week 14 Mouse models for pancreatic cancer and diabetes
<br>Week 15 Liver cancer (HCC) and Colorectal cancer model
<br>Week 16 Injury and tissue regeneration
<br>Week 17 Mouse models in Neuroscience and brain mapping
<br>Week 18 Final report
<br>
<br>
<br>六、成績考核(Evaluation)
<br>Attendance (10%), Discussion/Seminar (30%),Mid-term (30%) and Final report 
<br>(30%).
<br>