一、課程說明(Course Desc<x>ription) 
<br>透過實驗操作，讓學生熟悉生物化學中各主要分子(如醣類、脂肪、胺基酸和蛋白質等)的特性，以及生化研 
<br>究中經常使用到的實驗方法與技術(如透析、電泳、色層分析、酵素的分離與檢測等)。 
<br>
<br>二、指定用書(Text Books) 
<br>生物化學實驗講義(在第一堂課時發給) 
<br>
<br>三、教學方式(Teaching Method) 
<br>學生在老師及助教的指導下﹐依照實驗講義操作實驗 ﹐並繳交報告。 
<br>
<br>四、教學進度(Syllabus) 
<br>
<br>Week Experiments 
<br>1 Introduction and grouping 
<br>2 pH indicators and spectrophotometry 
<br>Part I Studies of biomolecules 
<br>3 Determination of carbohydrate and vitamin 
<br>4 Lipid Aggregation in Water and permeability of membrane 
<br>6 Assay of amino acids 
<br>7 Isoelectric points and absorption spectra of amino acids 
<br>8 Quantification and precipitation of proteins 
<br>Part II Basic experimental techniques 
<br>9 Dialysis 
<br>10 Column chromatography 
<br>11 Thin la<x>yer chromatography 
<br>12 Electrophoresis 
<br>Part III Enzyme kinetics 
<br>13 Photosynthesis, chloroplast isolation and activity test 
<br>14 Characterization of an enzyme, acid phosphatase 
<br>15 Kinetic properties of acid phosphatase 
<br>16 Final examination; Cleaning and check-out 
<br>
<br>五、成績考核(Evaluation) 
<br>
<br>1.Laboratory note/report (40%) 
<br>2.Final examination (30%) 
<br>3.Lab performance evaluated by teaching assistants (30%) 
<br>
<br>
<br>