Syllabus for Special Topics on Live Cell Imaging

Lecture-1: Introduction to live cell imaging
-- General introduction
Lecture-2: The choice of microscope setting
$713; Introduce current modules of conventional microscopes
$713; Epifluorescence microscope
$713; Deconvolution microscope
$713; Confocal-based microscope
Lecture-3: The choice of protein labeling
$713; Introduce fluorescent proteins
$713; Novel developed commercial tags for multiple imaging
Lecture-4: Visualize the organelles in live cells
$713; Organelle markers
$713; Fluorescence dyes used for living cells
Lecture-5: Cell cycle application on live cell imaging
$713; Interphase versus mitosis
$713; Mitotic progression
$713; Chromosome dynamics
$713; Cytokinesis
Lecture-6: Monitor intracellular trafficking
$713; Dynamic analysis of macromolecules
Lecture-7: Monitor protein-protein interaction
$713; Colocalization
$713; FRET
$713; FLIM
Lecture-8: Mid-term exam
Lecture-9: Practical training - set up the experiment and image analysis
Lecture-10: Practical training - cell cycle progression
Lecture-11: Practical training - mitotic progression
Lecture-12: Practical training – endocytosis/phagocytosis
Lecture-13: Practical training – membrane fusion
Lecture-14: Practical training – protein trafficking
Lecture-15: Final Exam