一、課程說明(Course Desc<x>ription) 
<br>This is an experimental course for students interested in the application of 
<br>biology and biomedical materials in biomedical engineering related sciences. 
<br>It 
<br>will teach the basic technique for conducting research in the fields of 
<br>biomedical engineering sciences. 
<br>
<br>課程設計涵蓋三個面向：
<br>I 生醫材料：
<br>(a)	生化小分子與蛋白質大分子材料之製備與性質分析
<br>(b)	生物高分子與奈米粒子之製備與性質分析
<br>II 生醫分析：
<br>(a)	材料與蛋白質之耦合分析
<br>(b)	材料與細胞之耦合分析
<br>(c)	細胞分析
<br>課程精神為生醫分析可經由生醫材料的製備以進行生物系統的探索。
<br>
<br>二、指定用書(Text Books) 
<br>Handouts will be provided in the class. 
<br>
<br>三、參考書籍(References) 
<br>四、教學方式(Teaching Method) 
<br>Laboratory 
<br>
<br>五、教學進度(Syllabus)
<br>部分時段採分組上課，詳細分組時段於第一周上課宣布。
<br>
<br>課程內容包含：                                                             
<br>1. Introduction:
<br>2. Preparation of the substrate for beta-galactosidasepart part I (半乳糖水解脢
<br>受體之
<br>製備I) (俞鐘山)                                                                                 
<br>3. Preparation of the substrate for beta-galactosidasepart part II (半乳糖水解
<br>脢受體
<br>之製備II)) (俞鐘山)                                                                           
<br>4. Protein Analysis: size-exclusion chromatography and SDS-PAGE (蛋白質分析方
<br>法：管柱
<br>層析與膠體電泳) (張建文)
<br>5. Encapsulation and sustained release of proteins in biodegradable particles 
<br>(生
<br>物可降解高分子/蛋白質粒子製備與釋放試驗) (張建文)
<br>6. Preparation and swelling characterization of pH-sensitive hydrogels (酸鹼應
<br>答型水
<br>膠製備與膨潤性質分析) (邱信程)
<br>7. Synthesis of Ferrofluid and the Properties under External Magnet(磁流體的製
<br>備與在
<br>磁場下的作用) (胡尚秀) 
<br>8. MTT assay for cytotoxicity (以MTT方法偵測奈米粒子細胞毒性) (莊淳宇)
<br>9. PCR/electrophoresis for gene determination (鏈鎖聚合%238;反應/電泳偵測基因) ((莊
<br>淳宇)
<br>10. Synthesis and characterization of gold nanoparticle (金奈米粒子的合成與鑑定) 
<br>(黃郁
<br>棻)
<br>11. Study of protein-gold nanoparticle conjugates (奈米金於蛋白質吸附和電性分析之
<br>應用) 
<br>(黃郁棻)
<br>12. The application of flow cytometry for cell phenotyping (以細胞流速儀分析細胞
<br>表面抗
<br>原）(江啟勳)
<br>13. Cell phagocytosis assay (細胞吞噬行為分析）(江啟勳).
<br>六、成績考核(Evaluation) 
<br>Lab performance 
<br>Lab report 
<br>
<br>七、可連結之網頁位址 
<br>NTHU e learning system 
<br>