一、課程說明(Course Description)
This is an experimental course for students interested in the application of
biology and biomedical materials in biomedical engineering related sciences.
It
will teach the basic technique for conducting research in the fields of
biomedical engineering sciences.
課程設計涵蓋三個面向:
I 生醫材料:
(a) 生化小分子與蛋白質大分子材料之製備與性質分析
(b) 生物高分子與奈米粒子之製備與性質分析
II 生醫分析:
(a) 材料與蛋白質之耦合分析
(b) 材料與細胞之耦合分析
(c) 細胞分析
課程精神為生醫分析可經由生醫材料的製備以進行生物系統的探索。
二、指定用書(Text Books)
Handouts will be provided in the class.
三、參考書籍(References)
四、教學方式(Teaching Method)
Laboratory
五、教學進度(Syllabus)
部分時段採分組上課,詳細分組時段於第一周上課宣布。
課程內容包含:
1. Introduction:
2. Preparation of the substrate for beta-galactosidasepart part I (半乳糖水解脢
受體之
製備I) (俞鐘山)
3. Preparation of the substrate for beta-galactosidasepart part II (半乳糖水解
脢受體
之製備II)) (俞鐘山)
4. Protein Analysis: size-exclusion chromatography and SDS-PAGE (蛋白質分析方
法:管柱
層析與膠體電泳) (張建文)
5. Encapsulation and sustained release of proteins in biodegradable particles
(生
物可降解高分子/蛋白質粒子製備與釋放試驗) (張建文)
6. Preparation and swelling characterization of pH-sensitive hydrogels (酸鹼應
答型水
膠製備與膨潤性質分析) (邱信程)
7. Synthesis of Ferrofluid and the Properties under External Magnet(磁流體的製
備與在
磁場下的作用) (胡尚秀)
8. MTT assay for cytotoxicity (以MTT方法偵測奈米粒子細胞毒性) (莊淳宇)
9. PCR/electrophoresis for gene determination (鏈鎖聚合%238;反應/電泳偵測基因) ((莊
淳宇)
10. Synthesis and characterization of gold nanoparticle (金奈米粒子的合成與鑑定)
(黃郁
棻)
11. Study of protein-gold nanoparticle conjugates (奈米金於蛋白質吸附和電性分析之
應用)
(黃郁棻)
12. The application of flow cytometry for cell phenotyping (以細胞流速儀分析細胞
表面抗
原)(江啟勳)
13. Cell phagocytosis assay (細胞吞噬行為分析)(江啟勳).
六、成績考核(Evaluation)
Lab performance
Lab report
七、可連結之網頁位址
NTHU e learning system