一、課程說明	
<br>This course will cover several modern biophysical tools for protein-ligand 
<br>interactions. These 
<br>tools include isothermal titration calorimetry (ITC), microscale thermophoresis 
<br>(MST), surface 
<br>plasmon resonance (SPR), fluorescence spectrometry, and uv-vis spectrometry with 
<br>emphasis on 
<br>the advantages and limitations of each technique. There will be a demo of ITC, 
<br>MST, and 
<br>fluorescence spectrometry in class.
<br>
<br>二、指定用書	
<br>Biophysical Tools for Biologists, Volume One: In Vitro Techniques. Edited by Dr. 
<br>John J. Correia 
<br>and Dr. H. William Detrich, III (2008) ISBN: 978-0-12-372520-2 
<br>
<br>三、參考書籍	
<br>1. Principles of Fluorescence Spectroscopy. 3rd edition, Joseph R. Lakowicz 
<br>(2011) ISBN: 
<br>0387312781
<br>2. Handbook of Single Molecule Fluorescence Spectroscopy. Christopher Gell, 
<br>David Brockwell, 
<br>Alastair Smith (2006) ISBN: 978-0198529422
<br>
<br>四、教學方式	
<br>互動式教學，鼓勵學生提問和發表意見。
<br>
<br>五、教學進度	
<br>March: basic principles of protein-ligand interactions
<br>April: ITC and SDC
<br>May: SPR; basic principles and applications of fluorescence spectroscopy
<br>June: fluorescence single-molecule measurements
<br>
<br>六、成績考核	
<br>課堂參與(20%)，每週期刊口頭報告(40%)，每週作業(40%)
<br>
<br>七、講義位址
<br>http://lms.nthu.edu.tw/
<br>