111 學年第一學期 分子生物學實驗課程
<br>任課老師	 E-mail	                  Office	分機
<br>藍忠昱	cylan@life.nthu.edu.tw  生科一館405室	42473
<br>李政昇	cslee@life.nthu.edu.tw  生科一館427室	42755
<br>任課助教			
<br>黃詩婷	sthuang@life.nthu.edu.tw LSII R328C	35859
<br>羅巧玲	cllo@life.nthu.edu.tw	LSII R328C	35859
<br>上課地點	生科二館321室		
<br>上課時間	每周四 13:20~17:20		
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<br>Week	Date	                 Syllabus	                   Professor
<br>01	9/15
<br>	        Check in and equipment introduction 	            藍忠昱/李政昇
<br>02	9/22	Introduction of bacteria culture	                藍忠昱
<br>03	9/29	Small scale preparation of bacterial plasmid DNA	藍忠昱
<br>04	10/6	Isolation of yeast genomic DNA
<br>                Spectroscopic analysis of bacterial plasmid DNA and 
<br>                yeast genomic DNA	                                藍忠昱
<br>05	10/13	Restriction digestion of bacterial plasmid DNA and 
<br>                yeast genomic DNA Agarose gel electrophoresis	        藍忠昱
<br>06	10/20	Isolation of yeast total RNA
<br>                Spectroscopic analysis of RNA (Nano-Drop)	        藍忠昱
<br>07	10/27	DNase treatment of yeast RNAs	藍忠昱
<br>08	11/3	Agarose gel electrophoresis: yeast total RNA 
<br>                (with or without DNase treatment) 
<br>                Reverse transc<x>ription (first strand cDNA synthesis)     李政昇
<br>09	11/10	Polymerase Chain Reaction (using plasmid DNA/cDNA 
<br>                and genomic DNA as a template) & Agarose gel 
<br>                electrophoresis: PCR product	                        李政昇
<br>10	11/17	Restriction digestion of cloning vector (plasmid DNA) 
<br>                and insert (PCR product) 
<br>                Agarose gel electrophoresis (Gel extraction of 
<br>                cloning vector and insert)
<br>                Spectroscopic analysis and construction of 
<br>                recombinant DNA (ligation)	                        李政昇
<br>11	11/24	Preparation of competent E. coli host cells
<br>                Transformation - Calcium chloride method & 
<br>                electroporation	                                        李政昇  
<br>12	12/1	Colony PCR & Agarose gel electrophoresis &
<br>                Preparation of sodium dodecyl sulfate-polyacrylamide 
<br>                gel electrophoresis (SDS-PAGE)	                        李政昇
<br>13	12/8	Verification of recombinant DNA and Protein ex<x>pression
<br>                SDS-polyacrylamide gel electrophoresis	                李政昇
<br>14	12/15	Transfer membrane and immunoblotting 
<br>                Western blotting analysis	                        李政昇
<br>15	12/22	Final exam and check out	                    藍忠昱/李政昇
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<br>評分標準
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<br>平常成績(預習報告、遲到缺席、上課態度及穿著等): 30% 
<br>成果報告(兩份): 40%
<br>期末考: 20%
<br>操作考: 10%
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