一、課程說明(Course Desc<x>ription)
<br>In this course we will learn: 1) recombinant DNA technology; 2) procedure of 
<br>generation of transgenic and gene knock mice; 3) the molecular mechanism of 
<br>gene gain/loss of function in mouse transgenic model; 4) conventional and 
<br>conditional transgenic gene ex<x>pression models; 5) conventional and 
<br>conditional 
<br>gene KO models and Cre-Lox technology; 6) applications of mouse models in 
<br>developmental biology research; 7) established mouse models in human disease 
<br>research. We will review about how transgenic animals are generated and what 
<br>recombinant DNA technologies are involved. The well-established disease models 
<br>will be introduced in class and discussed, including the rationale and 
<br>application of transgenic models in developmental biology, physiology and 
<br>disease 
<br>related research.
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<br>二、指定用書(Text Books)
<br>Selected papers
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<br>三、參考書籍(References)
<br>(1)Methods in Enzymology Vol. 476. Guide to Techniques in Mouse Development, 
<br>Part A: Mice, Embryos, and Cells, 2nd Edition. Edited by Paul M. Wassarman and 
<br>Philippe M. Soriano.
<br>(2)Regulation of Gene ex<x>pression: Molecular Mechanisms. Gary H. Perdew, John 
<br>P. Vanden Heuvel, and Jeffrey M. Peters. 2006. 
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<br>四、教學方式(Teaching Method)
<br>Lecture/ Seminar /Discussion
<br>
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<br>五、教學進度(Syllabus)
<br>Week 1	Introduction and principle of transgenic mouse models 
<br>Week 2	Promoters and tissue specific ex<x>pression
<br>Week 3  Doxycycline and Tamoxifen inducible system 
<br>Week 4  Loss of function technique (I) : Technical knockout: Antisense RNAi
<br>Week 5  Physical gene targeting (KO): Homologous recombination in ES 
<br>        cells 
<br>Week 6	Loss of function technique (II):Physical gene targeting gene KnockIn  
<br>Week 7	Conditional gene knockout with nuclease: CRE-LoxP, CRSIPR/Cas9 (I)
<br>Week 8  Lineage tracing in Developmental Biology Research  
<br>Week 9	mid-term
<br>Week 10	Gene trapping strategy and Mutagenesis
<br>Week 11 Mouse models in Neuroscience and brain mapping
<br>Week 12	Genetic and chemical induced lung cancer models
<br>Week 13	Prostate cancer models: Tramp and Lady
<br>Week 14	Mouse models for pancreatic cancer and diabetes
<br>Week 15	Liver cancer (HCC) and Colorectal cancer model
<br>Week 16 Injury and tissue regeneration
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<br>六、成績考核(Evaluation)
<br>Attendance (10%), Discussion/Seminar (30%),Mid-term (30%) and Final report 
<br>(30%).
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<br>七、講義位址
<br>    https://eeclass.nthu.edu.tw/
<br>