一、課程說明(Course Desc<x>ription)
<br>This class will introduce the major concepts and methods in homologous 
<br>recombination of chromosomes. The students will learn how DNA double strand breaks 
<br>are repaired by homologous recombination mainly in budding yeast. Both conceptual 
<br>background in yeast genetics and detailed molecular approaches to analyze 
<br>chromosomes will be covered.
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<br>二、指定用書(Text Books)
<br>DNA Recombination Editor(s): Tsubouchi, Hideo ISBN: 978-1-61779-129-1
<br>Humana Press
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<br>三、參考書籍(References) 
<br>Selected papers from high profile journals
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<br>四、教學方式(Teaching Method)
<br>All students are required to read all assigned chapters and papers and then 
<br>participate in classroom discussion.
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<br>五、教學進度(Syllabus)
<br>1-4. Methods to Study Mitotic Homologous Recombination and Genome Stability
<br>5-8. Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks 
<br>and Telomere Ends
<br>9-10. Characterization of Meiotic Recombination Initiation Sites Using Pulsed-
<br>Field Gel Electrophoresis .
<br>11-12. Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using 
<br>Single-Stranded DNA
<br>13-14. Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes
<br>15-18. Molecular Assays to Investigate Chromatin Changes During DNA Double-Strand 
<br>Break Repair in Yeast
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<br>六、成績考核(Evaluation)
<br>Class discussion: 40%. Assigned presentation: 40%. Attendance: 20%.
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<br>七、採用下列何項 AI 使用規則 (Indicate which of the following options you use to 
<br>manage student use of the AI)
<br>有條件開放，請註明如何使用生成式AI於課程產出 Conditionally open; please specify how 
<br>generative AI will be used in course output
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<br>八、講義位址
<br>http://eeclass.nthu.edu.tw
<br>