1. Course Desc<x>ription
<br>This class will introduce the theory, practice, and application of single 
<br>particle 
<br>cryo-EM. This course will discuss practical and conceptual approaches to 
<br>structure 
<br>determination using these techniques, as well as covering a wide range of state-
<br>of-the-art applications of cryo-EM in the biological sciences. 
<br>
<br>2. Text Books
<br>N/A
<br>
<br>3. References
<br>Selected papers from high profile journals
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<br>4. Teaching Method
<br>All students are required to read all assigned papers and participate in 
<br>classroom 
<br>discussion.
<br>
<br>5. Syllabus
<br>This course encourages students to discuss publications from peer-reviewed 
<br>journals and presents their own experimental progress. Through oral presentations 
<br>and peer discussion, students will explore different aspects of the cryo-EM 
<br>field. 
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<br>Week 1. Historical perspective on structural biology methods
<br>Week 2. Unique capabilities of cryo-EM: Virus 
<br>Week 3. Unique capabilities of cryo-EM: Cytoskeletons
<br>Week 4. Unique capabilities of cryo-EM: Ion channel
<br>Week 5. Unique capabilities of cryo-EM: DNA-protein complex
<br>Week 6. Unique capabilities of cryo-EM: RNA-protein complex
<br>Week 7. Unique capabilities of cryo-EM: Membrane proteins I
<br>Week 8. Unique capabilities of cryo-EM: Membrane proteins II
<br>Week 9. Unique capabilities of cryo-EM: Polymers
<br>Week 10. Scarce samples
<br>Week 11. Compositionally heterogenous samples
<br>Week 12. Conformationally complex samples
<br>Week 13. Current limits of cryo-EM and its true potential
<br>Week 14. Tomography
<br>Week 15. Experimental reports from participants I
<br>Week 16. Experimental reports from participants II
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<br>6. Evaluation
<br>In class discussion: 50%
<br>Oral presentation: 50%
<br>